Key publications

Biomolecular Analysis

Haselberg R, de Jong GJ, Somsen GW.
Low-flow sheathless capillary electrophoresis-mass spectrometry for sensitive glycoform profiling of intact pharmaceutical proteins.
Anal. Chem. 2013; 85: 2289-2296

Kok MGM, Ruijken MMA, Swann JR, Wilson ID, Somsen GW, de Jong GJ.
Anionic metabolic profiling of urine from antibiotic-treated rats by capillary electrophoresis-mass spectrometry.
Anal. Bioanal. Chem. 2013; 405: 2585-2594

Haselberg R, de Jong GJ, Somsen GW.
Capillary electrophoresis-mass spectrometry for the analysis of biopharmaceuticals.
LC GC North America 2012; 30: 504-509

Haselberg R, Harmsen S, Dolman MEM, de Jong GJ, Kok RJ, Somsen GW.
Characterization of drug-lysozyme conjugates by sheathless capillary electrophoresis–time-of-flight mass spectrometry.
Anal. Chim. Acta 2011; 698: 77-83

de Kort BJ, ten Kate GA, de Jong GJ, Somsen GW.
Capillary electrophoresis with lamp-based wavelength-resolved fluorescence detection for the probing of protein conformational changes.
Anal. Chem. 2011; 83: 6060-6067

Hommerson P, Khan AM, de Jong GJ, Somsen GW.
Ionization techniques in capillary electrophoresis-mass spectrometry: principles, design and application.
Mass Spectrom. Rev. 2011; 30: 1096-1120

Bioactivity Screening Technologies

Heus F, Vonk F, Otvos RA, Bruyneel B, Smit AB, Lingeman H, Richardson M, Niessen WMA, Kool J.
An efficient analytical platform for on-line microfluidic profiling of neuroactive snake venoms towards nicotinic receptor affinity.
Toxicon 2013; 61: 112-124

Nijmeijer S, Vischer HF, Rudebeck AF; Fleurbaaij F, Falck D, Leurs R, Niessen WMA, Kool J.
Development of a profiling strategy for metabolic mixtures by combining chromatography and mass spectrometry with cell-based GPCR signaling.
J. Biomol. Screening 2012; 17: 1329-1338

Kool J, Rudebeck AF, Fleurbaaij F, Nijmeijer S, Falck D, Smits RA, Vischer HF, Leurs R, Niessen WMA.
High-resolution metabolic profiling towards G protein-coupled receptors: Rapid and comprehensive screening of histamine H4 receptor ligands.
J. Chromatogr. A 2012; 1259: 213–220

Falck D, de Vlieger JSB, Giera M, Honing M, Irth H, Niessen WMA, Kool J.
On-line electrochemistry-bioaffinity screening with parallel HR-LC-MS for the generation and characterization of modified p38α kinase inhibitors.
Anal. Bioanal. Chem. 2012; 403: 367-375

Kool J, Giera M, Irth H, Niessen WM.
Advances in mass spectrometry-based post-column bioaffinity profiling of mixtures.
Anal. Bioanal. Chem. 2011; 399: 2655-2668

Kool J, de Kloe G, Denker AD, van Altena K, Smoluch M, van Iperen D, Nahar TT, Limburg RJ, Niessen WM, Lingeman H, Leurs R, de Esch IJ, Smit AB, Irth H.
Nanofractionation spotter technology for rapid contactless and high-resolution deposition of LC eluent for further off-line analysis.
Anal. Chem. 2011; 83: 125-132

Analytics of Biomolecular Interactions

Zauner G, Selman MH, Bondt A, Rombouts Y, Blank D, Deelder AM, Wuhrer M.
Glycoproteomic analysis of antibodies.
Mol. Cell Proteomics 2013; 12: 856-865

Reusch D, Haberger M, Selman MH, Bulau P, Deelder AM, Wuhrer M., Engler N.
High-throughput workflow for IgG Fc-glycosylation analysis of biotechnological samples.
Anal. Biochem. 2013; 432: 82-89

Wuhrer M.
Glycomics using mass spectrometry.
Glycoconjugate J. 2013; 30: 11-22

Zauner G, Kozak R, Gardner R, Fernandes DL, Deelder AM, Wuhrer M.
Protein O-glycosylation analysis.
Biol. Chem. 2012; 393: 687-708

Kozak RP, Royle L, Gardner RA, Fernandes DL, Wuhrer M.
Suppression of peeling during the release of O-glycans by hydrazinolysis.
Anal. Biochem. 2012; 423: 119-128

Dall’Olio F, Vanhooren V, Chen CC, Slagboom PE, Wuhrer M, Franceschi C.
N-glycomic biomarkers of biological ageing and longevity: a link with inflammageing.
Ageing Res. Rev. 2012; 12: 685-698

Bio-analysis: Sample pretreatment and separation methods

Kloos DP, Lingeman H, Niessen WMA, Deelder AM, Giera M, Mayboroda OA.
Evaluation of different column chemistries for fast urinary metabolic profiling.
J. Chromatogr. B 2013; 927: 90-96

Kloos D, Derks RJ, Wijtmans M, Lingeman H, Mayboroda OA, Deelder AM, Niessen WM, Giera M.
Derivatization of the tricarboxylic acid cycle intermediates and analysis by online solid-phase extraction-liquid chromatography-mass spectrometry with positive-ion electrospray ionization.
J. Chromatogr. A 2012; 1232: 19-26

Giera M, Kloos DP; Raaphorst A, Mayboroda OA, Deelder AM, Lingeman H, Niessen WMA.
Mild and selective labeling of malondialdehyde with 2-aminoacridone: assessment of urinary malondialdehyde levels.
Analyst 2011; 136: 2763-2769